Appendix C: Simulation of M2AChR Dose-Response Relationship Data Using an Asymmetric Current Change Model

To try to understand the source of the variability in suppressed M2AChR cEC50 data, we performed an experiment in which we repeated the same concentration of ACh (0.3 μM) at various intervals throughout a dose-response experiment (see Figure 3.22). Current responses to the same dose of agonist increased on average throughout the course of the experiments. More importantly, this change was not constant: current changes appeared to be low at the early doses in the series, but increased at the half-way point of the series.